How did Dolly’s cloning take place?
By: Daniela Garcia (M)

Graphic by Luis Aguila (M)

 

The natural reproduction of a sheep is by a fertilized sperm of the male sheep that goes into the egg cell that contains chromosomes. The chromosomes of both, the sperm and the egg, combine to form a complete set of chromosome pairs. The cell divides forming an embryo, the offspring is now containing a combination of two sets of chromosomes (both parents). The offspring it is not quite identical to either parent but is genetically one of a kind.

Sheep Dolly was cloned by a Somatic Cell nuclear Transfer that is a very common technique to clone animal cells. Its concept is to take the cells of the animal, but not the ones of the sexual organs. Once they had the cell; they took the nucleus off the cell. They must have the somatic cell first, containing the complete set of chromosomes. Then, the cell is taken away from the animal. The somatic cell is saved in a medium that causes it to lose its character as an under cell. Then, the egg cell nucleus containing the DNA is removed. The egg cell and the somatic cell are combined using an electric pulse. The nucleus of the somatic cell, containing a full set of chromosomes pairs is transferred to the egg cell. The cell is transferred to the mother forming the embryo. Because the offspring received all of the chromosomes from the somatic cell, it is an exact clone of the somatic cell donor.

I learned the difference between the natural reproduction of the sheep and the way Sheep Dolly was cloned with a somatic cell nuclear transfer. If someone comes and asks me a question referred to the natural reproduction and the way they cloned Sheep Dolly, I could easily answer what I learned. I learned that natural reproduction is done through the steps already explained earlier. The first sheep was cloned by a somatic cell nuclear transfer.

Bibliography

• http://gslc.genetics.utah.edu/units/cloning/whatiscloning/
• http://www.ornl.gov/sci/techresources/Human_Genome/elsi/cloning.shtml